Genotype instability during long-term subculture of lymphoblastoid cell lines
نویسندگان
چکیده
منابع مشابه
Instability at Short Tandem Repeats in Lymphoblastoid Cell Lines
OBJECTIVES Epstein Barr virus (EBV)-transformed lymphoblastoid cell lines (LCLs) are a useful biological resource, however, genomic variations can happen during the generation and immortalization processes of LCLs. The purpose of this study was to identify genomic variations in LCL DNA compared with matched blood DNA using short tandem repeats (STRs) analysis. METHODS We analyzed 15 STRs with...
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Background: The aim of this study was to develop methods for cryopreservation and long-term maintenance of putative bovine embryonic stem cells (ESCs). Materials and Methods: Putative bovine ESC (bESC) lines (n=3) isolated in conventional medium were used to compare slow-freezing and vitrification. Results: After warming, vitrified cells (96.9%) demonstrated significantly (p<0.05) better surviv...
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The expression level for 15,887 transcripts in lymphoblastoid cell lines from 19 monozygotic twin pairs (10 male, 9 female) were analysed for the effects of genotype and sex. On an average, the effect of twin pairs explained 31% of the variance in normalized gene expression levels, consistent with previous broad sense heritability estimates. The effect of sex on gene expression levels was most ...
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We compared genotype data from the HumanExomeCore Array in peripheral blood mononuclear cells and low passage lymphoblastoid cell lines from the same 24 individuals to test for genotypic errors caused by the Epstein-Barr Virus transformation process. Genotype concordance across the 24 comparisons was 99.57% for unfiltered genotype data, and 99.63% following standard genotype quality control fil...
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Introduction Former experiments have shown an increase in number of unstable chromosomal aberrations in normal human fibroblast strains with ongoing culturing time of the populations [1, 2]. These de novo formed unstable aberrations are an indicator of genomic instability which is considered to be one important step towards carcinogenesis. In the same experiments we observed the accumulation of...
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ژورنال
عنوان ژورنال: Journal of Human Genetics
سال: 2012
ISSN: 1434-5161,1435-232X
DOI: 10.1038/jhg.2012.123